truseq stranded total rna library prep gold kit  (Illumina Inc)

Journal: Bioactive Materials

Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

doi: 10.1016/j.bioactmat.2026.01.047

Figure Lengend Snippet: Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

Article Snippet: The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA).

Techniques: Biomarker Discovery, Transfection, Expressing, Binding Assay, Fluorescence, Imaging, Flow Cytometry, In Vitro, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Immunoprecipitation Assay

Journal: Biomaterials Research

Article Title: Anti-senescence Effects of Nanovesicles Derived from Cluster of Differentiation-146-Positive Tonsil Mesenchymal Stem Cells via Modulation of the Tumor Protein 53 Pathway

doi: 10.34133/bmr.0371

Figure Lengend Snippet: In vitro analysis of CD146 + TMSC-NVs in a passage-associated cellular senescence model. (A) Morphological changes of human dermal fibroblasts (HDFs) after treatment with CD146 + TMSC-NVs (scale bar = 200 μm). (B) Senescence-associated β-galactosidase (SA-β-gal) assay after treatment with CD146 + TMSC-NVs (scale bar = 200 μm). (C) Cell proliferation test using Cell Counting Kit-8 (CCK-8) assay during treatment with CD146 + TMSC-NVs. (D) Quantitative data following colorization with the SA-β-gal-positive cells. (E to G) Quantitative data of the polymerase chain reaction for the mRNA expression of elastin, COL1, and HMOX1. Statistical significance is shown as the mean ± standard deviation (* P < 0.05, *** P < 0.001, **** P < 0.0001; ns, not significant).

Article Snippet: To demonstrate the components within NVs, miRNA profiling was conducted by Macrogen, Inc. following the manufacturer’s protocol for the TruSeq Stranded mRNA Library Prep Kit.

Techniques: In Vitro, Cell Counting, CCK-8 Assay, Polymerase Chain Reaction, Expressing, Standard Deviation